Taylor, Michael and Scurr, David and Lutolf, Matthias and Buttery, Lee D.K. and Zelzer, Mischa and Alexander, Morgan R. (2015) 3D chemical characterization of frozen hydrated hydrogels using ToF-SIMS with argon

Taylor, Michael and Scurr, David and Lutolf, Matthias and Buttery, Lee and Zelzer, Mischa and Alexander, Morgan (2015) 3D chemical characterization of frozen hydrated hydrogels using ToF-SIMS with argon cluster

Multivariate analysis of 3D ToF-SIMS images: method validation and application to cultured neuronal networks.

Advanced data analysis tools are crucial for the application of ToF-SIMS analysis to biological samples. Here, we demonstrate that by using a training set approach principal components analysis (PCA) can be performed on large 3D ToF-SIMS images of neuronal cell cultures. The method readily provides access to sample component information and significantly improves the images' signal-to-noise rat...

Polymer Microarrays for High Throughput Discovery of Biomaterials

The discovery of novel biomaterials that are optimized for a specific biological application is readily achieved using polymer microarrays, which allows a combinatorial library of materials to be screened in a parallel, high throughput format (1). Herein is described the formation and characterization of a polymer microarray using an on-chip photopolymerization technique (2). This involves mixi...

Characterization of sample preparation methods of NIH/3T3 fibroblasts for ToF-SIMS analysis

The information that is obtained from single cells during time-of-flight secondary ion mass spectrometry (ToF-SIMS) analysis is influenced by the method that was used to prepare the cells. The removal of extracellular media before analysis is necessary, but the rinsing technique should not damage the plasma membrane of the cell. The presence of intracellular salts reduced the secondary ion yiel...

Strategies for MCR image analysis of large hyperspectral data-sets

Polymer microarrays are a key enabling technology for high throughput materials discovery. In this study, multivariate image analysis, specifically multivariate curve resolution (MCR), is applied to the hyperspectral time of flight secondary ion mass spectroscopy (ToF-SIMS) data from eight individual microarray spots. Rather than analysing the data individually, the data-sets are collated and a...